noun
trademark gag; signature routine
Used for a comedian's well-known joke, story, or performance piece that they are associated with. Often implies a personal staple that audiences expect.
See also: ネタ
あの芸人は、いつも同じ持ちネタで笑いを取る。
That comedian always gets laughs with the same trademark gag.
彼の持ちネタは、電車のアナウンスのモノマネだ。
His signature routine is an impression of train announcements.