noun which may take the genitive case particle 'no', noun
once in a lifetime; once in one's life
Emphasizes that something is a rare, possibly unique opportunity or experience. Often used with nouns like 機会 (chance) or 体験 (experience).
It's a once-in-a-lifetime chance, so I definitely don't want to miss it.
For her, it was the biggest stage of her life.
Compound of 一生 (whole life) and 一度 (once). The meaning is transparent from its components.