noun which may take the genitive case particle 'no', noun
once in a lifetime; once in one's life
Emphasizes that something is a rare, possibly unique opportunity or experience. Often used with nouns like 機会 (chance) or 体験 (experience).
一生一度のチャンスだから、絶対に逃したくない。
It's a once-in-a-lifetime chance, so I definitely don't want to miss it.
彼女にとって、それは一生一度の大舞台だった。
For her, it was the biggest stage of her life.
Compound of 一生 (whole life) and 一度 (once). The meaning is transparent from its components.